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cd36 mediated absorption pathway  (MedChemExpress)


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    MedChemExpress cd36 mediated absorption pathway
    Docosahexaenoic acid (DHA) enhances the proliferation of grass carp myoblasts through the cluster of differentiation 36 <t>(CD36)-mediated</t> uptake mechanism. (A and B) The relative mRNA expression levels of proliferation-related genes (cyclin D1 and cyclin E) in myoblasts treated with DHA for 24 h. (C) Percentage of 5-ethynyl-2′-deoxyuridine (EdU)-positive myoblastsrelative to the total myoblasts. (D) EdU (red fluorescence) and Hoechst (blue fluorescence, nuclei) staining. Scale bar, 200 μm. Control, untreated; DHA, treated with 50 μmol/L DHA; DHA + SSO, co-treated with 50 μmol/L DHA and 200 μmol/L SSO. SSO = sulfosuccinimidyl oleate sodium (a CD36 inhibitor). P -value less than 0.05 indicates a significant difference, n = 3.
    Cd36 Mediated Absorption Pathway, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36+mediated+absorption+pathway/Docosahexaenoic+acid/pmc13049527-181-9-28
    Average 95 stars, based on 56 article reviews
    cd36 mediated absorption pathway - by Bioz Stars, 2026-09
    95/100 stars

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    1) Product Images from "PPARα-CD36-CAV1-mediated docosahexaenoic acid (DHA) uptake promotes muscle fiber development in grass carp ( Ctenopharyngodon idellus )"

    Article Title: PPARα-CD36-CAV1-mediated docosahexaenoic acid (DHA) uptake promotes muscle fiber development in grass carp ( Ctenopharyngodon idellus )

    Journal: Animal Nutrition

    doi: 10.1016/j.aninu.2025.10.011

    Docosahexaenoic acid (DHA) enhances the proliferation of grass carp myoblasts through the cluster of differentiation 36 (CD36)-mediated uptake mechanism. (A and B) The relative mRNA expression levels of proliferation-related genes (cyclin D1 and cyclin E) in myoblasts treated with DHA for 24 h. (C) Percentage of 5-ethynyl-2′-deoxyuridine (EdU)-positive myoblastsrelative to the total myoblasts. (D) EdU (red fluorescence) and Hoechst (blue fluorescence, nuclei) staining. Scale bar, 200 μm. Control, untreated; DHA, treated with 50 μmol/L DHA; DHA + SSO, co-treated with 50 μmol/L DHA and 200 μmol/L SSO. SSO = sulfosuccinimidyl oleate sodium (a CD36 inhibitor). P -value less than 0.05 indicates a significant difference, n = 3.
    Figure Legend Snippet: Docosahexaenoic acid (DHA) enhances the proliferation of grass carp myoblasts through the cluster of differentiation 36 (CD36)-mediated uptake mechanism. (A and B) The relative mRNA expression levels of proliferation-related genes (cyclin D1 and cyclin E) in myoblasts treated with DHA for 24 h. (C) Percentage of 5-ethynyl-2′-deoxyuridine (EdU)-positive myoblastsrelative to the total myoblasts. (D) EdU (red fluorescence) and Hoechst (blue fluorescence, nuclei) staining. Scale bar, 200 μm. Control, untreated; DHA, treated with 50 μmol/L DHA; DHA + SSO, co-treated with 50 μmol/L DHA and 200 μmol/L SSO. SSO = sulfosuccinimidyl oleate sodium (a CD36 inhibitor). P -value less than 0.05 indicates a significant difference, n = 3.

    Techniques Used: Expressing, Fluorescence, Staining, Control

    Docosahexaenoic acid (DHA) promotes myofiber proliferation and development in muscle tissue via the PPARα-cluster of differentiation 36 (CD36)-mediated uptake pathway. (A) Site-directed mutagenesis analysis of PPARα binding sites on the pGL3.0-CD36 vector in human embryonic kidney 293T (HEK 293T) cells. (B) The mRNA expression levels of fatty acid absorption ( cd36 and cav1 ) in muscle tissue. (C) Muscle fiber diameter. (D) Muscle fiber density. (E) Representative hematoxylin and eosin (H&E) staining in muscle tissue. Scale bar, 100 μm. (F) The mRNA expression of muscle growth and development related genes ( myog , myod , myhc , mrf4 , and myf5 ) and fiber cell growth factor ( fgf6a and fgf6b ) in muscle tissue. Control, diet without DHA supplementation; DHA, diet supplemented with 0.5% DHA; DHA + GW6471, diet supplemented with 0.5% DHA and GW6471. GW6471 = peroxisome proliferator-activated receptor α inhibitor; Luc = luciferase. P -value less than 0.05 indicates a significant difference, n = 3.
    Figure Legend Snippet: Docosahexaenoic acid (DHA) promotes myofiber proliferation and development in muscle tissue via the PPARα-cluster of differentiation 36 (CD36)-mediated uptake pathway. (A) Site-directed mutagenesis analysis of PPARα binding sites on the pGL3.0-CD36 vector in human embryonic kidney 293T (HEK 293T) cells. (B) The mRNA expression levels of fatty acid absorption ( cd36 and cav1 ) in muscle tissue. (C) Muscle fiber diameter. (D) Muscle fiber density. (E) Representative hematoxylin and eosin (H&E) staining in muscle tissue. Scale bar, 100 μm. (F) The mRNA expression of muscle growth and development related genes ( myog , myod , myhc , mrf4 , and myf5 ) and fiber cell growth factor ( fgf6a and fgf6b ) in muscle tissue. Control, diet without DHA supplementation; DHA, diet supplemented with 0.5% DHA; DHA + GW6471, diet supplemented with 0.5% DHA and GW6471. GW6471 = peroxisome proliferator-activated receptor α inhibitor; Luc = luciferase. P -value less than 0.05 indicates a significant difference, n = 3.

    Techniques Used: Mutagenesis, Binding Assay, Plasmid Preparation, Expressing, Staining, Control, Luciferase

    Related Articles

    Expressing:

    Article Title: PPARα-CD36-CAV1-mediated docosahexaenoic acid (DHA) uptake promotes muscle fiber development in grass carp ( Ctenopharyngodon idellus )
    Article Snippet: Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.. To investigate whether DHA promotes myoblast proliferation via the CD36-mediated absorption pathway, this study utilized DHA (HY-B2167) and the CD36 inhibitor sulfosuccinimidyl oleate sodium (SSO; HY-112847A), both from MedChem Express LLC (Monmouth Junction, NJ, USA).. The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).

    Fluorescence:

    Article Title: PPARα-CD36-CAV1-mediated docosahexaenoic acid (DHA) uptake promotes muscle fiber development in grass carp ( Ctenopharyngodon idellus )
    Article Snippet: Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.. To investigate whether DHA promotes myoblast proliferation via the CD36-mediated absorption pathway, this study utilized DHA (HY-B2167) and the CD36 inhibitor sulfosuccinimidyl oleate sodium (SSO; HY-112847A), both from MedChem Express LLC (Monmouth Junction, NJ, USA).. The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).

    Staining:

    Article Title: PPARα-CD36-CAV1-mediated docosahexaenoic acid (DHA) uptake promotes muscle fiber development in grass carp ( Ctenopharyngodon idellus )
    Article Snippet: Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.. To investigate whether DHA promotes myoblast proliferation via the CD36-mediated absorption pathway, this study utilized DHA (HY-B2167) and the CD36 inhibitor sulfosuccinimidyl oleate sodium (SSO; HY-112847A), both from MedChem Express LLC (Monmouth Junction, NJ, USA).. The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).

    Control:

    Article Title: PPARα-CD36-CAV1-mediated docosahexaenoic acid (DHA) uptake promotes muscle fiber development in grass carp ( Ctenopharyngodon idellus )
    Article Snippet: Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.. To investigate whether DHA promotes myoblast proliferation via the CD36-mediated absorption pathway, this study utilized DHA (HY-B2167) and the CD36 inhibitor sulfosuccinimidyl oleate sodium (SSO; HY-112847A), both from MedChem Express LLC (Monmouth Junction, NJ, USA).. The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).

    Mutagenesis:

    Article Title: PPARα-CD36-CAV1-mediated docosahexaenoic acid (DHA) uptake promotes muscle fiber development in grass carp ( Ctenopharyngodon idellus )
    Article Snippet: Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.. To investigate whether DHA promotes myoblast proliferation via the CD36-mediated absorption pathway, this study utilized DHA (HY-B2167) and the CD36 inhibitor sulfosuccinimidyl oleate sodium (SSO; HY-112847A), both from MedChem Express LLC (Monmouth Junction, NJ, USA).. The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).

    Binding Assay:

    Article Title: PPARα-CD36-CAV1-mediated docosahexaenoic acid (DHA) uptake promotes muscle fiber development in grass carp ( Ctenopharyngodon idellus )
    Article Snippet: Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.. To investigate whether DHA promotes myoblast proliferation via the CD36-mediated absorption pathway, this study utilized DHA (HY-B2167) and the CD36 inhibitor sulfosuccinimidyl oleate sodium (SSO; HY-112847A), both from MedChem Express LLC (Monmouth Junction, NJ, USA).. The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).

    Plasmid Preparation:

    Article Title: PPARα-CD36-CAV1-mediated docosahexaenoic acid (DHA) uptake promotes muscle fiber development in grass carp ( Ctenopharyngodon idellus )
    Article Snippet: Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.. To investigate whether DHA promotes myoblast proliferation via the CD36-mediated absorption pathway, this study utilized DHA (HY-B2167) and the CD36 inhibitor sulfosuccinimidyl oleate sodium (SSO; HY-112847A), both from MedChem Express LLC (Monmouth Junction, NJ, USA).. The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).

    Luciferase:

    Article Title: PPARα-CD36-CAV1-mediated docosahexaenoic acid (DHA) uptake promotes muscle fiber development in grass carp ( Ctenopharyngodon idellus )
    Article Snippet: Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.Following centrifugation, the resulting cell pellet was resuspended in fresh medium and transferred to new flasks for continued passaging.. To investigate whether DHA promotes myoblast proliferation via the CD36-mediated absorption pathway, this study utilized DHA (HY-B2167) and the CD36 inhibitor sulfosuccinimidyl oleate sodium (SSO; HY-112847A), both from MedChem Express LLC (Monmouth Junction, NJ, USA).. The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).The myoblasts were divided into three groups: the control group, the DHA group (50 μmol/L DHA incubated alone for 24 h), and the DHA + SSO group (pre-incubated with 200 μmol/L CD36 inhibitor SSO for 4 h, followed by co-incubation with 50 μmol/L DHA for 24 h).



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    cd36 mediated absorption pathway  (MedChemExpress)
    95
    MedChemExpress cd36 mediated absorption pathway
    Docosahexaenoic acid (DHA) enhances the proliferation of grass carp myoblasts through the cluster of differentiation 36 <t>(CD36)-mediated</t> uptake mechanism. (A and B) The relative mRNA expression levels of proliferation-related genes (cyclin D1 and cyclin E) in myoblasts treated with DHA for 24 h. (C) Percentage of 5-ethynyl-2′-deoxyuridine (EdU)-positive myoblastsrelative to the total myoblasts. (D) EdU (red fluorescence) and Hoechst (blue fluorescence, nuclei) staining. Scale bar, 200 μm. Control, untreated; DHA, treated with 50 μmol/L DHA; DHA + SSO, co-treated with 50 μmol/L DHA and 200 μmol/L SSO. SSO = sulfosuccinimidyl oleate sodium (a CD36 inhibitor). P -value less than 0.05 indicates a significant difference, n = 3.
    Cd36 Mediated Absorption Pathway, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36+mediated+absorption+pathway/Docosahexaenoic+acid/pmc13049527-181-9-28
    Average 95 stars, based on 1 article reviews
    cd36 mediated absorption pathway - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Docosahexaenoic acid (DHA) enhances the proliferation of grass carp myoblasts through the cluster of differentiation 36 (CD36)-mediated uptake mechanism. (A and B) The relative mRNA expression levels of proliferation-related genes (cyclin D1 and cyclin E) in myoblasts treated with DHA for 24 h. (C) Percentage of 5-ethynyl-2′-deoxyuridine (EdU)-positive myoblastsrelative to the total myoblasts. (D) EdU (red fluorescence) and Hoechst (blue fluorescence, nuclei) staining. Scale bar, 200 μm. Control, untreated; DHA, treated with 50 μmol/L DHA; DHA + SSO, co-treated with 50 μmol/L DHA and 200 μmol/L SSO. SSO = sulfosuccinimidyl oleate sodium (a CD36 inhibitor). P -value less than 0.05 indicates a significant difference, n = 3.

    Journal: Animal Nutrition

    Article Title: PPARα-CD36-CAV1-mediated docosahexaenoic acid (DHA) uptake promotes muscle fiber development in grass carp ( Ctenopharyngodon idellus )

    doi: 10.1016/j.aninu.2025.10.011

    Figure Lengend Snippet: Docosahexaenoic acid (DHA) enhances the proliferation of grass carp myoblasts through the cluster of differentiation 36 (CD36)-mediated uptake mechanism. (A and B) The relative mRNA expression levels of proliferation-related genes (cyclin D1 and cyclin E) in myoblasts treated with DHA for 24 h. (C) Percentage of 5-ethynyl-2′-deoxyuridine (EdU)-positive myoblastsrelative to the total myoblasts. (D) EdU (red fluorescence) and Hoechst (blue fluorescence, nuclei) staining. Scale bar, 200 μm. Control, untreated; DHA, treated with 50 μmol/L DHA; DHA + SSO, co-treated with 50 μmol/L DHA and 200 μmol/L SSO. SSO = sulfosuccinimidyl oleate sodium (a CD36 inhibitor). P -value less than 0.05 indicates a significant difference, n = 3.

    Article Snippet: To investigate whether DHA promotes myoblast proliferation via the CD36-mediated absorption pathway, this study utilized DHA (HY-B2167) and the CD36 inhibitor sulfosuccinimidyl oleate sodium (SSO; HY-112847A), both from MedChem Express LLC (Monmouth Junction, NJ, USA).

    Techniques: Expressing, Fluorescence, Staining, Control

    Docosahexaenoic acid (DHA) promotes myofiber proliferation and development in muscle tissue via the PPARα-cluster of differentiation 36 (CD36)-mediated uptake pathway. (A) Site-directed mutagenesis analysis of PPARα binding sites on the pGL3.0-CD36 vector in human embryonic kidney 293T (HEK 293T) cells. (B) The mRNA expression levels of fatty acid absorption ( cd36 and cav1 ) in muscle tissue. (C) Muscle fiber diameter. (D) Muscle fiber density. (E) Representative hematoxylin and eosin (H&E) staining in muscle tissue. Scale bar, 100 μm. (F) The mRNA expression of muscle growth and development related genes ( myog , myod , myhc , mrf4 , and myf5 ) and fiber cell growth factor ( fgf6a and fgf6b ) in muscle tissue. Control, diet without DHA supplementation; DHA, diet supplemented with 0.5% DHA; DHA + GW6471, diet supplemented with 0.5% DHA and GW6471. GW6471 = peroxisome proliferator-activated receptor α inhibitor; Luc = luciferase. P -value less than 0.05 indicates a significant difference, n = 3.

    Journal: Animal Nutrition

    Article Title: PPARα-CD36-CAV1-mediated docosahexaenoic acid (DHA) uptake promotes muscle fiber development in grass carp ( Ctenopharyngodon idellus )

    doi: 10.1016/j.aninu.2025.10.011

    Figure Lengend Snippet: Docosahexaenoic acid (DHA) promotes myofiber proliferation and development in muscle tissue via the PPARα-cluster of differentiation 36 (CD36)-mediated uptake pathway. (A) Site-directed mutagenesis analysis of PPARα binding sites on the pGL3.0-CD36 vector in human embryonic kidney 293T (HEK 293T) cells. (B) The mRNA expression levels of fatty acid absorption ( cd36 and cav1 ) in muscle tissue. (C) Muscle fiber diameter. (D) Muscle fiber density. (E) Representative hematoxylin and eosin (H&E) staining in muscle tissue. Scale bar, 100 μm. (F) The mRNA expression of muscle growth and development related genes ( myog , myod , myhc , mrf4 , and myf5 ) and fiber cell growth factor ( fgf6a and fgf6b ) in muscle tissue. Control, diet without DHA supplementation; DHA, diet supplemented with 0.5% DHA; DHA + GW6471, diet supplemented with 0.5% DHA and GW6471. GW6471 = peroxisome proliferator-activated receptor α inhibitor; Luc = luciferase. P -value less than 0.05 indicates a significant difference, n = 3.

    Article Snippet: To investigate whether DHA promotes myoblast proliferation via the CD36-mediated absorption pathway, this study utilized DHA (HY-B2167) and the CD36 inhibitor sulfosuccinimidyl oleate sodium (SSO; HY-112847A), both from MedChem Express LLC (Monmouth Junction, NJ, USA).

    Techniques: Mutagenesis, Binding Assay, Plasmid Preparation, Expressing, Staining, Control, Luciferase